Image- and model-based analysis of constitutive properties of cellular structures

Evgeny Gladilin; Roland Eils

doi:10.1117/12.839668

9 March 2010 Image- and model-based analysis of constitutive properties of cellular structures

Evgeny Gladilin, Roland Eils

Author Affiliations +

Proceedings Volume 7626, Medical Imaging 2010: Biomedical Applications in Molecular, Structural, and Functional Imaging; 76261D (2010) https://doi.org/10.1117/12.839668
Event: SPIE Medical Imaging, 2010, San Diego, California, United States

Abstract

Determination of constitutive properties of cells is important for quantitative description of cellular mechanics. Existing approaches to mechanical cell manipulation are based on experimental techniques that do not allow unsupervised analysis of large number of cells and/or probing of intracellular structures that are not directly exposed to external loads. Alternatively, mechanical behavior of cellular matter can be studied in time-series of microscopic images. In this work, we present an image- and model-based framework for determination of constitutive properties of living cells. Our experimental studies demonstrate application of this approach for quantitative analysis of cellular mechanics on the basis of image data assessed by different experimental techniques, including microplate stretching, optical stretching and contactless cellular deformation induction using cytoskeleton-disrupting drugs.

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Evgeny Gladilin and Roland Eils "Image- and model-based analysis of constitutive properties of cellular structures", Proc. SPIE 7626, Medical Imaging 2010: Biomedical Applications in Molecular, Structural, and Functional Imaging, 76261D (9 March 2010); https://doi.org/10.1117/12.839668

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