A microfluidic system for semicontinuous bead-based biosensing platforms

Hye Won Kim; Young Man Kim; Sung Keun Yoo; Sang Youl Yoon; Jong Hyun Lee; Sung Yang

doi:10.1117/12.853098

25 November 2009 A microfluidic system for semicontinuous bead-based biosensing platforms

Hye Won Kim, Young Man Kim, Sung Keun Yoo, Sang Youl Yoon, Jong Hyun Lee, Sung Yang

Proceedings Volume 7634, Optical Sensors and Biophotonics; 76340I (2009) https://doi.org/10.1117/12.853098
Event: Asia Communications and Photonics, 2009, Shanghai, Shanghai , China

Abstract

Conventional bead-based micro immunoassays do not guarantee high reliability and repeatability due to the trapping mechanisms of the systems which cause inconsistency in trapping of cytometric beads. This paper presents a new beadbased micro immunoassay system for semicontinuous assays with high reliability and accuracy in cytometric bead trapping by using the hole-patterned microstructures. Trapping test showed the assembly of single layer of bead array in ordered fashion. In addition that, the μ-immunoassay exhibits that the fluorescence intensity linearly increases in proportion to Biotin-4-Fluorescin (B4F) concentration and a 10 pg/mL appears to be the lowest detectable concentration for B4F after incubation time of 5 minutes.

Citation Download Citation

Hye Won Kim, Young Man Kim, Sung Keun Yoo, Sang Youl Yoon, Jong Hyun Lee, and Sung Yang "A microfluidic system for semicontinuous bead-based biosensing platforms", Proc. SPIE 7634, Optical Sensors and Biophotonics, 76340I (25 November 2009); https://doi.org/10.1117/12.853098

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