Automatic procedure for non-coplanar aberration compensation in lensless Fourier transform digital holography

Huakun Cui; Dayong Wang; Yunxin Wang; Changgeng Liu; Jie Zhao; Yan Li

doi:10.1117/12.865688

11 October 2010 Automatic procedure for non-coplanar aberration compensation in lensless Fourier transform digital holography

Huakun Cui, Dayong Wang, Yunxin Wang, Changgeng Liu, Jie Zhao, Yan Li

Author Affiliations +

Proceedings Volume 7656, 5th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment; 76560R (2010) https://doi.org/10.1117/12.865688
Event: 5th International Symposium on Advanced Optical Manufacturing and Testing Technologies, 2010, Dalian, China

Abstract

As the lensless Fourier transform digital holography is applied into the microscopic phase-contrast imaging on the live cells, the motion of the cells will lead to the non-coplanarity phenomena between the object and the reference source. This could result in the imaging aberration. An effective and robust autofocus procedure based on the phase distribution is presented in the paper. With the initial measurement of the distance between the reference source and the hologram, the optimum parameters corresponding to the phase-contrast image can be achieved by a single hologram, combined with the linearity fitting. The lensless Fourier transform digital holographic system is built and the experiments on the phase-contrast imaging of the live cervical carcinoma cells are performed. Finally, the good experiment results are demonstrated. Both the theoretical analysis and the experimental investigation verify the feasibility and validity of the automatic procedure for the non-coplanar aberration compensation.

Citation Download Citation

Huakun Cui, Dayong Wang, Yunxin Wang, Changgeng Liu, Jie Zhao, and Yan Li "Automatic procedure for non-coplanar aberration compensation in lensless Fourier transform digital holography", Proc. SPIE 7656, 5th International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment, 76560R (11 October 2010); https://doi.org/10.1117/12.865688

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