Development of a microfluidic system for measuring HIV-1 viral load

Shuqi Wang; Alexander Ip; Feng Xu; Francoise F. Giguel; SangJun Moon; Altug Akay; Daniel R. Kuritzkes; Utkan Demirci

doi:10.1117/12.853132

5 May 2010 Development of a microfluidic system for measuring HIV-1 viral load

Shuqi Wang, Alexander Ip, Feng Xu, Francoise F. Giguel, SangJun Moon, Altug Akay, Daniel R. Kuritzkes, Utkan Demirci

Proceedings Volume 7666, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX; 76661H (2010) https://doi.org/10.1117/12.853132
Event: SPIE Defense, Security, and Sensing, 2010, Orlando, Florida, United States

Abstract

The World Health Organization (WHO) is rapidly expanding antiretroviral treatment (ART) in sub-Saharan countries. However, virological failure of ART is rarely monitored due to the lack of affordable and sustainable viral load assays suitable for resource-limited settings. Here, we report a prototype of a rapid virus detection method based on microfluidic technologies. In this method, HIV-1 particles from 10 μL whole blood were captured by anti-gp120 antibody coated on the microchannel surface and detected by dual fluorescence signals under microscopy. Next, captured HIV-1 particles were counted using the free software, ImageJ (http://rsbweb.nih.gov/ij/). This rapid HIV-1 detection method has potential to be further developed for viral load monitoring at resource-limited settings.

Citation Download Citation

Shuqi Wang, Alexander Ip, Feng Xu, Francoise F. Giguel, SangJun Moon, Altug Akay, Daniel R. Kuritzkes, and Utkan Demirci "Development of a microfluidic system for measuring HIV-1 viral load", Proc. SPIE 7666, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense IX, 76661H (5 May 2010); https://doi.org/10.1117/12.853132

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