Biophotonics determination of 6-benzylaminopurine (6-BAP) plant growth regulator using OFRR biosensor

Gilmo Yang; Sukwon Kang; Kangjin Lee; Giyoung Kim; Jaeryong Son; Changyeun Mo

doi:10.1117/12.852096

20 April 2010 Biophotonics determination of 6-benzylaminopurine (6-BAP) plant growth regulator using OFRR biosensor

Gilmo Yang, Sukwon Kang, Kangjin Lee, Giyoung Kim, Jaeryong Son, Changyeun Mo

Proceedings Volume 7676, Sensing for Agriculture and Food Quality and Safety II; 76760L (2010) https://doi.org/10.1117/12.852096
Event: SPIE Defense, Security, and Sensing, 2010, Orlando, Florida, United States

Abstract

The identification of pesticide and 6-benzylaminopurine (6-BAP) plant growth regulator was carried out using a label-free opto-fluidic ring resonator (OFRR) biosensor. The OFRR sensing platform is a recent advancement in opto-fluidic technology that integrates photonic sensing technology with microfluidics. It features quick detection time, small sample volume, accurate quantitative and kinetic results. The most predominant advantage of the OFRR integrated with microfluidics is that we can potentially realize the multi-channel and portable biosensor that detects numerous analytes simultaneously. Antisera for immunoassay were raised in rabbits against the 6-BAP-BSA conjugate. Using the immunization protocol and unknown cytokinin reacting with same antibody, comparable sensitivity and specificity were obtained. 6-BAP antibody was routinely used for cytokinin analysis. A sensitive and simple OFRR method with a good linear relationship was developed for the determination of 6-BAP. The detection limit was also examined. The biosensor demonstrated excellent reproducibility when periodically exposed to 6-BAP.

Citation Download Citation

Gilmo Yang, Sukwon Kang, Kangjin Lee, Giyoung Kim, Jaeryong Son, and Changyeun Mo "Biophotonics determination of 6-benzylaminopurine (6-BAP) plant growth regulator using OFRR biosensor", Proc. SPIE 7676, Sensing for Agriculture and Food Quality and Safety II, 76760L (20 April 2010); https://doi.org/10.1117/12.852096

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