Aptamer-based surface plasmon fibre sensor for thrombin detection

T. Allsop; D. Nagel; R. Neal; E. M. Davies; C. Mou; P. Bond; S. Rehman; K. Kalli; D. J. Webb; P. Calverhouse; M. Mascini; I. Bennion

doi:10.1117/12.854334

17 May 2010 Aptamer-based surface plasmon fibre sensor for thrombin detection

T. Allsop, D. Nagel, R. Neal, E. M. Davies, C. Mou, P. Bond, S. Rehman, K. Kalli, D. J. Webb, P. Calverhouse, M. Mascini, I. Bennion

Author Affiliations +

Proceedings Volume 7715, Biophotonics: Photonic Solutions for Better Health Care II; 77151C (2010) https://doi.org/10.1117/12.854334
Event: SPIE Photonics Europe, 2010, Brussels, Belgium

Abstract

A series of surface plasmonic fibre devices were fabricated using multiple coatings deposited on a lapped section of a single mode fibre and post-fabrication UV laser irradiation processing with a phase mask, producing a surface relief grating structure. These devices showed high spectral sensitivity in the aqueous index regime ranging up to 4000 nm/RIU for wavelength and 800 dB/RIU for intensity. The devices were then coated with human thrombin binding aptamer. Several concentrations of thrombin in buffer solution were made, ranging from 1nM to 1μM. All the concentrations were detectable by the devices demonstrating that sub-nM concentrations may be monitored.

Citation Download Citation

T. Allsop, D. Nagel, R. Neal, E. M. Davies, C. Mou, P. Bond, S. Rehman, K. Kalli, D. J. Webb, P. Calverhouse, M. Mascini, and I. Bennion "Aptamer-based surface plasmon fibre sensor for thrombin detection", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151C (17 May 2010); https://doi.org/10.1117/12.854334

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