Effects of fluorescent lighting on in vitro micropropagation of Lemna minor

Kollawat Somsri; Pataradawn Pinyopich; Waleed S. Mohammed

doi:10.1117/12.861349

19 May 2010 Effects of fluorescent lighting on in vitro micropropagation of Lemna minor

Kollawat Somsri, Pataradawn Pinyopich, Waleed S. Mohammed

Proceedings Volume 7743, Southeast Asian International Advances in Micro/Nanotechnology; 77430G (2010) https://doi.org/10.1117/12.861349
Event: Southeast Asian International Advances in Micro/Nano-technology, 2010, Bangkok, Thailand

Abstract

The vegetative in vitro propagation of Lemna minor stain SING-4 exposed to two different types of fluorescent light sources, Philips TLD 36W/54 and Toshiba FL40T8BRF/36, was studied. The liquid culture medium contained 4.43gl^-1 phytohormone-free full-strength Murashige & Skoog (MS) basal medium with vitamins, 30gl^-1 sucrose, and 1gl^-1 MES. The results showed that both plant cultures had undergone normal asexual reproduction with an exponential increase trend. Cultures exposed to Toshiba FL40T8BRF/36 reproduced at a slightly faster rate while expressing significantly greener foliage (leaf color chart shade No.8), which indicates the presence of more chlorophyll, than cultures exposed to Philips TLD 36W/54 (leaf color chart shade No.4). The data obtained from our experiment reveals that light emitted from Toshiba FL40T8BRF/36 produces healthier and higher quality cultures.

Citation Download Citation

Kollawat Somsri, Pataradawn Pinyopich, and Waleed S. Mohammed "Effects of fluorescent lighting on in vitro micropropagation of Lemna minor", Proc. SPIE 7743, Southeast Asian International Advances in Micro/Nanotechnology, 77430G (19 May 2010); https://doi.org/10.1117/12.861349

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