The microstructure of mandibular bone grafts and three-dimensional cell clusters

Sarper Gürel; Corinne Unold; Hans Deyhle; Georg Schulz; Sebastian Kühl; Belma Saldamli; Jutta Tübel; Rainer Burgkart; Felix Beckmann; Bert Müller

doi:10.1117/12.859437

20 September 2010 The microstructure of mandibular bone grafts and three-dimensional cell clusters

Sarper Gürel, Corinne Unold, Hans Deyhle, Georg Schulz, Sebastian Kühl, Belma Saldamli, Jutta Tübel, Rainer Burgkart, Felix Beckmann, Bert Müller

Author Affiliations +

Proceedings Volume 7804, Developments in X-Ray Tomography VII; 78041G (2010) https://doi.org/10.1117/12.859437
Event: SPIE Optical Engineering + Applications, 2010, San Diego, California, United States

Abstract

During the last decade, several tissues and biomaterials for medical applications in replacing bony tissues have been developed. Three-dimensional cell clusters and mandibular bone grafts are two distinct examples of these developments. The characterization of the complex three-dimensional structures, however, is still mainly restricted on the twodimensional analysis of histological slices. The present paper examines the quantitative analysis of mandibular bone grafts and three-dimensional cell clusters on the basis of synchrotron radiation-based micro computed tomography measurements. An automated search of pre-defined microstructures through component labeling is applied to the real datasets in order to identify features that reside independently from other components. The examples demonstrate three levels of complexity: rather large pieces of bone augmentation material that touch each other, individual adipocytes difficult to automatically segment in a wet cluster and osmium-stained adipocyte exhibiting higher X-ray absorption than the surrounding tissue. Although the structures of interest such as the cells can be labeled, de-clustering of the components requires the incorporation of erosion and dilation algorithms.

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Sarper Gürel, Corinne Unold, Hans Deyhle, Georg Schulz, Sebastian Kühl, Belma Saldamli, Jutta Tübel, Rainer Burgkart, Felix Beckmann, and Bert Müller "The microstructure of mandibular bone grafts and three-dimensional cell clusters", Proc. SPIE 7804, Developments in X-Ray Tomography VII, 78041G (20 September 2010); https://doi.org/10.1117/12.859437

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