Nanopore formation in neuroblastoma cells following ultrashort electric pulse exposure

Caleb C Roth; Jason A Payne; Gerald J. Wilmink; Bennett L. Ibey

doi:10.1117/12.875777

18 February 2011 Nanopore formation in neuroblastoma cells following ultrashort electric pulse exposure

Caleb C Roth, Jason A Payne, Gerald J. Wilmink, Bennett L. Ibey

Proceedings Volume 7883, Photonic Therapeutics and Diagnostics VII; 788354 (2011) https://doi.org/10.1117/12.875777
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Ultrashort or nanosecond electrical pulses (USEP) cause repairable damage to the plasma membranes of cells through formation of nanopores. These nanopores are able to pass small ions such as sodium, calcium, and potassium, but remain impermeable to larger molecules like trypan blue and propidium iodide. What remains uncertain is whether generation of nanopores by ultrashort electrical pulses can inhibit action potentials in excitable cells. In this paper, we explored the sensitivity of excitable cells to USEP using Calcium Green AM 1 ester fluorescence to measure calcium uptake indicative of nanopore formation in the plasma membrane. We determined the threshold for nanopore formation in neuroblastoma cells for three pulse parameters (amplitude, pulse width, and pulse number). Measurement of such thresholds will guide future studies to determine if USEP can inhibit action potentials without causing irreversible membrane damage.

Citation Download Citation

Caleb C Roth, Jason A Payne, Gerald J. Wilmink, and Bennett L. Ibey "Nanopore formation in neuroblastoma cells following ultrashort electric pulse exposure", Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 788354 (18 February 2011); https://doi.org/10.1117/12.875777

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