Photobiomodulatory effects of He-Ne laser on excision wounds

Vijendra Prabhu; Satish B. S. Rao; Pramod Kumar; Lakshmi Rao; Krishna K. Mahato

doi:10.1117/12.874814

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17 February 2011 Photobiomodulatory effects of He-Ne laser on excision wounds

Vijendra Prabhu, Satish B. S. Rao, Pramod Kumar, Lakshmi Rao, Krishna K. Mahato

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Proceedings Volume 7887, Mechanisms for Low-Light Therapy VI; 78870H (2011) https://doi.org/10.1117/12.874814
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Presently, great importance has been given to Low Level Laser Therapy (LLLT) with the intent of promoting wound healing process. The present study was aimed to investigate the promotive effect of LLLT on full thickness excision wounds in Swiss albino mice using optical fiber probe based light device. Circular wounds of diameter 15 mm were illuminated with single exposure of various laser doses 1, 2, 3, 4, 6, 8 and 10 J/cm² along with appropriate controls. Further, an optimal dose of 2 J/cm² was applied to excision wounds at different post-wounding treatment schedules (0, 24 h and 48 h) to explicate the relations between treatment schedule and its tissue regeneration potential. Wound area, mean wound healing time along with hydroxyproline and glucosamine levels from wound ground tissue was assessed to evaluate the resultant photobiostimulatory outcome. Histological analysis was performed on day 10 of post-wounding. A significant increase in hydroxyproline (P< 0.001) and glucosamine levels (P< 0.01) were observed in 2 J/cm² irradiation group, which was also substantiated by histological findings. In conclusion, the present study demonstrated that the immediate irradiation of 2 J/cm² dose following wounding hasten the healing process compared to the unilluminated control.

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Vijendra Prabhu, Satish B. S. Rao, Pramod Kumar, Lakshmi Rao, and Krishna K. Mahato "Photobiomodulatory effects of He-Ne laser on excision wounds", Proc. SPIE 7887, Mechanisms for Low-Light Therapy VI, 78870H (17 February 2011); https://doi.org/10.1117/12.874814

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