Endoscopic detection of murine colonic dysplasia using a novel fluorescence-labeled peptide

Sharon J. Miller; Bishnu P. Joshi; Adam Gaustad; Eric R. Fearon; Thomas D. Wang

doi:10.1117/12.873530

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22 February 2011 Endoscopic detection of murine colonic dysplasia using a novel fluorescence-labeled peptide

Sharon J. Miller, Bishnu P. Joshi, Adam Gaustad, Eric R. Fearon, Thomas D. Wang

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Proceedings Volume 7893, Endoscopic Microscopy VI; 789303 (2011) https://doi.org/10.1117/12.873530
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Current endoscopic screening does not detect all pre-malignant (dysplastic) colorectal mucosa, thus requiring the development of more sensitive, targeted techniques to improve detection. The presented work utilizes phage display to identify a novel peptide binder to colorectal dysplasia in a CPC;Apc mouse model. A wide-field, small animal endoscope capable of fluorescence excitation (450-475 nm) identified polyps via white light and also collected fluorescence images (510 nm barrier filter) of peptide binding. The peptide bound ~2-fold greater to the colonic adenomas when compared to the control peptide. We have imaged fluorescence-labeled peptide binding in vivo that is specific towards distal colonic adenomas.

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Sharon J. Miller, Bishnu P. Joshi, Adam Gaustad, Eric R. Fearon, and Thomas D. Wang "Endoscopic detection of murine colonic dysplasia using a novel fluorescence-labeled peptide", Proc. SPIE 7893, Endoscopic Microscopy VI, 789303 (22 February 2011); https://doi.org/10.1117/12.873530

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