Fused oblique incidence reflectometry and confocal fluorescence microscopy

Matthew D. Risi; Andrew R. Rouse; Arthur F. Gmitro

doi:10.1117/12.875974

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22 February 2011 Fused oblique incidence reflectometry and confocal fluorescence microscopy

Matthew D. Risi, Andrew R. Rouse, Arthur F. Gmitro

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Proceedings Volume 7893, Endoscopic Microscopy VI; 78930F (2011) https://doi.org/10.1117/12.875974
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Confocal microendoscopy provides real-time high resolution cellular level images via a minimally invasive procedure, but relies on exogenous fluorophores, has a relatively limited penetration depth (100 μm) and field of view (700 μm), and produces a high rate of detailed information to the user. A new catheter based multi-modal system has been designed that combines confocal imaging and oblique incidence reflectometry (OIR), which is a non-invasive method capable of rapidly extracting tissue absorption, μa, and reduced scattering, μ's, spectra from tissue. The system builds on previous developments of a custom slit-scan multi-spectral confocal microendoscope and is designed to rapidly switch between diffuse spectroscopy and confocal fluorescence imaging modes of operation. An experimental proof-of-principle catheter has been developed that consists of a fiber bundle for traditional confocal fluorescence imaging and a single OIR source fiber which is manually redirected at +/- 26 degrees. Diffusely scattered light from each orientation of the source fiber is collected via the fiber bundle, with a frame of data representing spectra collected at a range of distances from the OIR source point. Initial results with intralipid phantoms show good agreement to published data over the 550-650 nm spectral range. We successfully imaged and measured the optical properties of rodent cardiac muscle.

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Matthew D. Risi, Andrew R. Rouse, and Arthur F. Gmitro "Fused oblique incidence reflectometry and confocal fluorescence microscopy", Proc. SPIE 7893, Endoscopic Microscopy VI, 78930F (22 February 2011); https://doi.org/10.1117/12.875974

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