Imaging sub-nanomolar concentrations through more than five centimeters of tissue with time-domain diffuse fluorescence tomography?

Kenneth M. Tichauer; Frederic Leblond; Robert Holt; Fadi El-Ghussein; Brian W. Pogue

doi:10.1117/12.874801

17 February 2011 Imaging sub-nanomolar concentrations through more than five centimeters of tissue with time-domain diffuse fluorescence tomography?

Kenneth M. Tichauer, Frederic Leblond, Robert Holt, Fadi El-Ghussein, Brian W. Pogue

Author Affiliations +

Proceedings Volume 7896, Optical Tomography and Spectroscopy of Tissue IX; 78962U (2011) https://doi.org/10.1117/12.874801
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The superior sensitivity and dynamic range of photodetection using time-correlated single-photon counting (TCSPC) technology can extend the diameter of specimens that can be imaged with fluorescence molecular tomography (FMT) and extend the minimum fluorescence concentration that can be reconstructed. To test these limits, a multi-modal system combining microcomputed tomography (microCT) and time-domain TCSPC FMT was used to image a 5 cm-diameter tissue-simulating cylindrical phantom containing an inclusion of various concentrations of AlexaFluor 647, mixed with 1% intralipid in water. This was repeated at lower concentrations for a 2.5 cm-diameter cylindrical phantom. Fluorescence tomography images were reconstructed using the structural information obtained from the microCT (outer surface of the interrogated sample) as prior information for light transport modeling. Results are presented showing that the location of the fluorescent inclusion can be reconstructed down to nanomolar concentrations in the 5 cm phantom and down to sub-nanomolar concentrations in the 25 mm phantom. The ability to reconstruct fluorescence images of these phantoms demonstrates that an unprecedented level of sensitivity can be achieved with time-domain TCSPC fluorescence tomography allowing this technology to be used for applications involving animals larger than mice as well as for applications where limited contrast is available.

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Kenneth M. Tichauer, Frederic Leblond, Robert Holt, Fadi El-Ghussein, and Brian W. Pogue "Imaging sub-nanomolar concentrations through more than five centimeters of tissue with time-domain diffuse fluorescence tomography?", Proc. SPIE 7896, Optical Tomography and Spectroscopy of Tissue IX, 78962U (17 February 2011); https://doi.org/10.1117/12.874801

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KEYWORDS

Luminescence

Imaging systems

Optical properties

Fluorescence tomography

Data modeling

Transmittance

Fluorescence spectroscopy

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