Rotating wall vessel system designed for fluorescent imaging

Tristan J. Tayag; S. Dan Dimitrijevich; Lauren C. Del Gallego; Pankaj Kumar

doi:10.1117/12.873091

17 February 2011 Rotating wall vessel system designed for fluorescent imaging

Tristan J. Tayag, S. Dan Dimitrijevich, Lauren C. Del Gallego, Pankaj Kumar

Proceedings Volume 7897, Optical Interactions with Tissue and Cells XXII; 789718 (2011) https://doi.org/10.1117/12.873091
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Fluorescent imaging of cells and tissues cultured within a rotating wall vessel bioreactor offers quantitative assessment of the 3-dimensional aggregation of cells into tissue constructs. We present the design of a rotating wall vessel system optimized for real-time fluorescent analysis. The modulation transfer function of our system is found to be superior to the commercially-available vessel used in previous fluorescence imaging studies. We demonstrate dynamic fluorescent imaging of DAPI-stained porcine pancreatic islets.

Citation Download Citation

Tristan J. Tayag, S. Dan Dimitrijevich, Lauren C. Del Gallego, and Pankaj Kumar "Rotating wall vessel system designed for fluorescent imaging", Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 789718 (17 February 2011); https://doi.org/10.1117/12.873091

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