Do large fluorescent particles enhance the modulation efficiency of ultrasound-modulated fluorescence?

Yuan Liu; Baohong Yuan; Joseph Vignola

doi:10.1117/12.874906

22 February 2011 Do large fluorescent particles enhance the modulation efficiency of ultrasound-modulated fluorescence?

Yuan Liu, Baohong Yuan, Joseph Vignola

Proceedings Volume 7899, Photons Plus Ultrasound: Imaging and Sensing 2011; 789934 (2011) https://doi.org/10.1117/12.874906
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The question of whether particle size affects modulation efficiency, defined as the ratio of ultrasound-modulated fluorescence (UMF) signal to DC (direct current) signal, of the fluorescence emission from four different sized fluorescent particles was investigated experimentally. The four particles are streptavidin-conjugated Alexa Fluo 647 (~5 nm in diameter) and three carboxylate-modified fluorescent microspheres (FM) with different diameters of 0.02, 0.2, and 1.0 μm. Modulation efficiency was evaluated as a function of the fluorophore size and fluorophore concentration. The modulation efficiency was improved about two times when the size of the fluorescent particles is increased from 5 nm to 1 μm. This result implies that using large fluorescence particles can slightly improve the modulation efficiency but the improvement is limited.

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Yuan Liu, Baohong Yuan, and Joseph Vignola "Do large fluorescent particles enhance the modulation efficiency of ultrasound-modulated fluorescence?", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 789934 (22 February 2011); https://doi.org/10.1117/12.874906

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KEYWORDS

Modulation

Particles

Luminescence

Ultrasonography

Atmospheric particles

Optical amplifiers

Fourier transforms

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