Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy

YongKeun Park; Catherine Best; Thorsten Auth; Nir S. Gov; Samuel Safran; Gabriel Popescu

doi:10.1117/12.874768

22 February 2011 Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy

YongKeun Park, Catherine Best, Thorsten Auth, Nir S. Gov, Samuel Safran, Gabriel Popescu

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Proceedings Volume 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 79021M (2011) https://doi.org/10.1117/12.874768
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We have quantitatively and systemically measured the morphologies and dynamics of fluctuations in human RBC membranes using a full-field laser interferometry technique that accurately measures dynamic membrane fluctuations. We present conclusive evidence that the presence of adenosine 5'-triphosphate (ATP) facilitates nonequilibrium dynamic fluctuations in the RBC membrane and that these fluctuations are highly correlated with specific regions in the biconcave shape of RBCs. Spatial analysis reveals that these nonequilibrium membrane fluctuations are enhanced at the scale of the spectrin mesh size. Our results indicate the presence of dynamic remodeling in the RBC membrane cortex powered by ATP, which results in nonequilibrium membrane fluctuations.

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YongKeun Park, Catherine Best, Thorsten Auth, Nir S. Gov, Samuel Safran, and Gabriel Popescu "Metabolic remodeling of the human red blood cell membrane measured by quantitative phase microscopy", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021M (22 February 2011); https://doi.org/10.1117/12.874768

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