Infrared spectroscopic imaging of kidney tumor tissue

V. Sablinskas; G. Steiner; E. Koch; J. Ceponkus; M. Pucetaite; S. Strazdaite; V. Urboniene; F. Jankevicius

doi:10.1117/12.875258

22 February 2011 Infrared spectroscopic imaging of kidney tumor tissue

V. Sablinskas, G. Steiner, E. Koch, J. Ceponkus, M. Pucetaite, S. Strazdaite, V. Urboniene, F. Jankevicius

Proceedings Volume 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 790221 (2011) https://doi.org/10.1117/12.875258
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Infrared spectroscopic imaging of cancerous kidney tissue was performed by means of FTIR microscopy. The spectra of thin tissue cryosections were collected with 64x64 MCT FPA detector and imaging area was increased up to 5.4×5.4 mm by mapping by means of PC controlled x,y stage. Chemical images of the samples were constructed using statistical treatment of the raw spectra. Several unsupervised and supervised statistical methods were used. The imaging results are compared with results of the standard histopathological analysis. It was concluded that application of method of cluster analysis ensures the best contrast of the images. It was found that border between cancerous and normal tissues visible in the infrared spectroscopic image corresponds with the border visible in histopathological image. Closer examination of the infrared spectroscopic image reveals that small domains of cancerous cells are found beyond the border in areas distant from the border up to 3 mm. Such domains are not visible in the histopathological images. The smallest domains found in the infrared images are approx. 60 μm.

Citation Download Citation

V. Sablinskas, G. Steiner, E. Koch, J. Ceponkus, M. Pucetaite, S. Strazdaite, V. Urboniene, and F. Jankevicius "Infrared spectroscopic imaging of kidney tumor tissue", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 790221 (22 February 2011); https://doi.org/10.1117/12.875258

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