Simplified setup for imaging with digital holographic microscopy and enhanced quantitative phase contrast by osmotic stimulation of living cells

Björn Kemper; Sabine Przibilla; Christina E. Rommel; Angelika Vollmer; Steffi Ketelhut; Jürgen Schnekenburger; Gert von Bally

doi:10.1117/12.874020

11 February 2011 Simplified setup for imaging with digital holographic microscopy and enhanced quantitative phase contrast by osmotic stimulation of living cells

Björn Kemper, Sabine Przibilla, Christina E. Rommel, Angelika Vollmer, Steffi Ketelhut, Jürgen Schnekenburger, Gert von Bally

Author Affiliations +

Proceedings Volume 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII; 790404 (2011) https://doi.org/10.1117/12.874020
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Many interferometry-based quantitative phase contrast imaging techniques require the generation of a coherent reference wave, which results in a phase stability decrease and the demand for a precise adjustment of the intensity ratio between object and reference wave. Thus, investigations on a simplified digital holographic microscopy approach that avoids a separate reference wave were performed. Results from live cell investigations demonstrate the capability of the method for quantitative phase contrast imaging. In further experiments the modification of the intracellular refractive index distribution by osmotic stimulation was analyzed. Data from human pancreas tumor cells show that by choice of suitable buffer solutions live cell imaging with enhanced quantitative phase contrast is achieved.

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Björn Kemper, Sabine Przibilla, Christina E. Rommel, Angelika Vollmer, Steffi Ketelhut, Jürgen Schnekenburger, and Gert von Bally "Simplified setup for imaging with digital holographic microscopy and enhanced quantitative phase contrast by osmotic stimulation of living cells", Proc. SPIE 7904, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII, 790404 (11 February 2011); https://doi.org/10.1117/12.874020

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