Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD

Ryan A. Colyer; Giuseppe Scalia; Federica A. Villa; Fabrizio Guerrieri; Simone Tisa; Franco Zappa; Sergio Cova; Shimon Weiss; Xavier Michalet

doi:10.1117/12.874238

28 February 2011 Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD

Ryan A. Colyer, Giuseppe Scalia, Federica A. Villa, Fabrizio Guerrieri, Simone Tisa, Franco Zappa, Sergio Cova, Shimon Weiss, Xavier Michalet

Author Affiliations +

Proceedings Volume 7905, Single Molecule Spectroscopy and Imaging IV; 790503 (2011) https://doi.org/10.1117/12.874238
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Single-molecule spectroscopy is a powerful approach to measuring molecular properties such as size, brightness, conformation, and binding constants. Due to the low concentrations in the single-molecule regime, measurements with good statistical accuracy require long acquisition times. Previously we showed a factor of 8 improvement in acquisition speed using a custom-CMOS 8x1 SPAD array. Here we present preliminary results with a 64X improvement in throughput obtained using a liquid crystal on silicon spatial light modulator (LCOS-SLM) and a novel standard CMOS 1024 pixel SPAD array, opening the way to truly high-throughput single-molecule spectroscopy.

Citation Download Citation

Ryan A. Colyer, Giuseppe Scalia, Federica A. Villa, Fabrizio Guerrieri, Simone Tisa, Franco Zappa, Sergio Cova, Shimon Weiss, and Xavier Michalet "Ultra high-throughput single molecule spectroscopy with a 1024 pixel SPAD", Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 790503 (28 February 2011); https://doi.org/10.1117/12.874238

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