Subunit rotation in a single FoF1-ATP synthase in a living bacterium monitored by FRET

K. Seyfert; T. Oosaka; H. Yaginuma; S. Ernst; H. Noji; R. Iino; M. Börsch

doi:10.1117/12.873066

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28 February 2011 Subunit rotation in a single F_oF₁-ATP synthase in a living bacterium monitored by FRET

K. Seyfert, T. Oosaka, H. Yaginuma, S. Ernst, H. Noji, R. Iino, M. Börsch

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Proceedings Volume 7905, Single Molecule Spectroscopy and Imaging IV; 79050K (2011) https://doi.org/10.1117/12.873066
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

F_oF₁-ATP synthase is the ubiquitous membrane-bound enzyme in mitochondria, chloroplasts and bacteria which provides the 'chemical energy currency' adenosine triphosphate (ATP) for cellular processes. In Escherichia coli ATP synthesis is driven by a proton motive force (PMF) comprising a proton concentration difference ΔpH plus an electric potential ΔΨ across the lipid membrane. Single-molecule in vitro experiments have confirmed that proton-driven subunit rotation within F_oF₁-ATP synthase is associated with ATP synthesis. Based on intramolecular distance measurements by single-molecule fluorescence resonance energy transfer (FRET) the kinetics of subunit rotation and the step sizes of the different rotor parts have been unraveled. However, these experiments were accomplished in the presence of a PMF consisting of a maximum ΔpH ~ 4 and an unknown ΔΨ. In contrast, in living bacteria the maximum ΔpH across the plasma membrane is likely 0.75, and ΔΨ has been measured between -80 and -140 mV. Thus the problem of in vivo catalytic turnover rates, or the in vivo rotational speed in single F_oF₁-ATP synthases, respectively, has to be solved. In addition, the absolute number of functional enzymes in a single bacterium required to maintain the high ATP levels has to be determined. We report our progress of measuring subunit rotation in single F_oF₁-ATP synthases in vitro and in vivo, which was enabled by a new labeling approach for single-molecule FRET measurements.

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K. Seyfert, T. Oosaka, H. Yaginuma, S. Ernst, H. Noji, R. Iino, and M. Börsch "Subunit rotation in a single F_oF₁-ATP synthase in a living bacterium monitored by FRET", Proc. SPIE 7905, Single Molecule Spectroscopy and Imaging IV, 79050K (28 February 2011); https://doi.org/10.1117/12.873066

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