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11 February 2011In Situ formation of microstructures near live cells using spatially
structured near-infrared laser microbeam
Here, we report in situ formation of microstructures from the regular constituents of culture media near live cells
using spatially-structured near infrared (NIR) laser beam. Irradiation with the continuous wave (cw) NIR laser
microbeam for few seconds onto the regular cell culture media containing fetal bovine serum resulted in
accumulation of dense material inside the media as evidenced by phase contrast microscopy. The time to form the
phase dense material was found to depend on the laser beam power. Switching off the laser beam led to diffusion of
phase dark material. However, the proteins could be stitched together by use of carbon nanoparticles and continuous
wave (cw) Ti: Sapphire laser beam. Further, by use of spatially-structured beam profiles different structures near live
cells could be formed. The microfabricated structure could be held by the Gravito-optical trap and repositioned by
movement of the sample stage. Orientation of these microstructures was achieved by rotating the elliptical laser
beam profile. Thus, multiple microstructures were formed and organized near live cells. This method would enable
study of response of cells/axons to the immediate physical hindrance provided by such structure formation and also
eliminate the biocompatibility requirement posed on artificial microstructure materials.
Ninad Ingle,Ling Gu, andSamarendra K. Mohanty
"In Situ formation of microstructures near live cells using spatially
structured near-infrared laser microbeam", Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 79080I (11 February 2011); https://doi.org/10.1117/12.876009
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Ninad Ingle, Ling Gu, Samarendra K. Mohanty, "In situ formation of microstructures near live cells using spatially structured near-infrared laser microbeam," Proc. SPIE 7908, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 79080I (11 February 2011); https://doi.org/10.1117/12.876009