3D visualization and quantification of bone and teeth mineralization for the study of osteo/dentinogenesis in mice models

A. Marchadier; C. Vidal; S. Ordureau; R. Lédée; C. Léger; M. Young; M. Goldberg

doi:10.1117/12.878251

9 March 2011 3D visualization and quantification of bone and teeth mineralization for the study of osteo/dentinogenesis in mice models

A. Marchadier, C. Vidal, S. Ordureau, R. Lédée, C. Léger, M. Young, M. Goldberg

Author Affiliations +

Proceedings Volume 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging; 79650J (2011) https://doi.org/10.1117/12.878251
Event: SPIE Medical Imaging, 2011, Lake Buena Vista (Orlando), Florida, United States

Abstract

Research on bone and teeth mineralization in animal models is critical for understanding human pathologies. Genetically modified mice represent highly valuable models for the study of osteo/dentinogenesis defects and osteoporosis. Current investigations on mice dental and skeletal phenotype use destructive and time consuming methods such as histology and scanning microscopy. Micro-CT imaging is quicker and provides high resolution qualitative phenotypic description. However reliable quantification of mineralization processes in mouse bone and teeth are still lacking. We have established novel CT imaging-based software for accurate qualitative and quantitative analysis of mouse mandibular bone and molars. Data were obtained from mandibles of mice lacking the Fibromodulin gene which is involved in mineralization processes. Mandibles were imaged with a micro-CT originally devoted to industrial applications (Viscom, X8060 NDT). 3D advanced visualization was performed using the VoxBox software (UsefulProgress) with ray casting algorithms. Comparison between control and defective mice mandibles was made by applying the same transfer function for each 3D data, thus allowing to detect shape, colour and density discrepencies. The 2D images of transverse slices of mandible and teeth were similar and even more accurate than those obtained with scanning electron microscopy. Image processing of the molars allowed the 3D reconstruction of the pulp chamber, providing a unique tool for the quantitative evaluation of dentinogenesis. This new method is highly powerful for the study of oro-facial mineralizations defects in mice models, complementary and even competitive to current histological and scanning microscopy appoaches.

Citation Download Citation

A. Marchadier, C. Vidal, S. Ordureau, R. Lédée, C. Léger, M. Young, and M. Goldberg "3D visualization and quantification of bone and teeth mineralization for the study of osteo/dentinogenesis in mice models", Proc. SPIE 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging, 79650J (9 March 2011); https://doi.org/10.1117/12.878251

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Bone

Teeth

Visualization

Volume rendering

Microscopy

3D image processing

3D visualizations

Show All Keywords

Keywords/Phrases

Search In:

Publication Years