Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement

Laurel M. Burk; Kohan Wang; Eunice Kang; Mauricio Rojas; Monte Willis; Yueh Z. Lee; Jianping Lu; Otto Zhou

doi:10.1117/12.878168

9 March 2011 Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement

Laurel M. Burk, Kohan Wang, Eunice Kang, Mauricio Rojas, Monte Willis, Yueh Z. Lee, Jianping Lu, Otto Zhou

Author Affiliations +

Proceedings Volume 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging; 79651N (2011) https://doi.org/10.1117/12.878168
Event: SPIE Medical Imaging, 2011, Lake Buena Vista (Orlando), Florida, United States

Abstract

We demonstrate the application of our cardiac- and respiratory-gated carbon nanotube (CNT) micro-CT system by evaluating murine myocardial infarction models with a delayed contrast enhancement technique. Myocardial infarction was induced in 8 wild-type male mice. The ischemia reperfusion model was achieved by surgical occlusion of the LAD artery for 30 minutes followed by 24 hours of reperfusion. Free-breathing subjects were anesthetized with isoflurane during imaging. Respiratory and cardiac signals were monitored externally to gate the scan. Micro-CT data was obtained at 50kV, 3mA cathode current for 15ms per projection. All images were acquired during end exhalation at either 0msec or 55msec after the R-wave (diastole or systole, respectively). Following administration of Omnipaque 300mgI/mL at 0.1ml/5g, images were obtained at 0msec after the R-wave. Fenestra VC was then administered at a 0.1ml/5g dose, followed by images 0 and 55msec after the R-wave. Hearts were then harvested, sliced 1mm thick and stained with TTC. All animals survived surgery and imaging; all demonstrated obvious delayed contrast enhancement in the left ventricular wall in Omnipaque images. Fenestra VC revealed cardiac functional changes quantified by low ejection fractions. All subjects demonstrated areas of myocardial infarct in the LAD distribution on both TTC staining and micro-CT imaging. CNT enabled gated cardiac micro-CT imaging demonstrates the ability to consistently identify areas of myocardial infarct in mice, providing a powerful tool for the study of cardiovascular biology. Further work is ongoing to streamline the imaging protocol and perform more quantitative analysis of the images.

Citation Download Citation

Laurel M. Burk, Kohan Wang, Eunice Kang, Mauricio Rojas, Monte Willis, Yueh Z. Lee, Jianping Lu, and Otto Zhou "Imaging of myocardial infarction using carbon nanotube micro-computed tomography and delayed contrast enhancement", Proc. SPIE 7965, Medical Imaging 2011: Biomedical Applications in Molecular, Structural, and Functional Imaging, 79651N (9 March 2011); https://doi.org/10.1117/12.878168

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