Raman spectroscopy of HIV-1 antigen and antibody

Pavel V. Zinin; Ningjie Hu; Lori E. Kamemoto; Qigui Yu; Anupam K. Misra; Shiv K. Sharma

doi:10.1117/12.887169

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26 May 2011 Raman spectroscopy of HIV-1 antigen and antibody

Pavel V. Zinin, Ningjie Hu, Lori E. Kamemoto, Qigui Yu, Anupam K. Misra, Shiv K. Sharma

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Proceedings Volume 8025, Smart Biomedical and Physiological Sensor Technology VIII; 80250D (2011) https://doi.org/10.1117/12.887169
Event: SPIE Defense, Security, and Sensing, 2011, Orlando, Florida, United States

Abstract

Raman spectra of anti-HIV-1 antibody, HIV-1 antigen (p24), and HIV-1 antibody-antigen complex have been measured in near-infrared and UV regions: 785 nm; 830 nm; and 244 nm laser excitations. The spectrum of the HIV-1 antigen was excited with an infrared laser and contains numerous Raman peaks. The most prominent peaks are broad bands at 1343, 1449, 1609 and 1655 cm^-1, which are characteristic of the Raman spectra of biological cells. The UV Raman spectrum of the HIV-1 antigen has a completely different structure. It has two strong peaks at 1613 cm^-1 and 1173 cm^-1. The peak at 1613 cm^-1 is associated with vibrations of the aromatic amino acids tyrosine (Tyr) and tryptophan (Try). The second strongest peak at 1173 cm^-1 is associated with the vibration of Tyr. The Raman peak pattern of the HIV-1 antigen-antibody complex is very similar to that of the HIV-1 antigen. The only difference is that the peak at 1007 cm^-1 of the Raman spectrum of the HIV-1 antigen-antibody complex is slightly enhanced compared to that of the HIV-1 antigen. This indicates that the peaks of the HIV-1 antigen dominate the Raman spectrum of the HIV-1 antigen-antibody complex.

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Pavel V. Zinin, Ningjie Hu, Lori E. Kamemoto, Qigui Yu, Anupam K. Misra, and Shiv K. Sharma "Raman spectroscopy of HIV-1 antigen and antibody", Proc. SPIE 8025, Smart Biomedical and Physiological Sensor Technology VIII, 80250D (26 May 2011); https://doi.org/10.1117/12.887169

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