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8 June 2011 Lensfree in-line holographic detection of bacteria
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Due to low light scattering, bacteria are difficult to detect using lensless imaging systems. In order to detect individual bacteria, we report a method based on a thin wetting film imaging that produces a micro-lens effect on top of each bacterium when the sample dries up. The imaging using a high-end CMOS sensor is combined with an in-line holographic reconstruction to improve positive detection rate up to 95% with micron-sized beads at high density of ~103 objects/mm2. The system allows detecting from single bacterium to densely packed objects (103 bacteria/μl) within 10μl sample. As an example, E.coli, Bacillus subtilis and Bacillus thuringiensis, has been successfully detected with strong signal to noise ratio across a 24mm2 field of view.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
V. Poher, C. P. Allier, J. G. Coutard, L. Hervé, and J. M. Dinten "Lensfree in-line holographic detection of bacteria", Proc. SPIE 8086, Advanced Microscopy Techniques II, 808619 (8 June 2011);

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