Simultaneous SHG and 2PEF imaging using a new type of selective markers

E. De Meulenaere; S. Van Cleuvenbergen; S. Psilodimitrakopoulos; J. Vanderleyden; P. Loza-Alvarez; K. Clays

doi:10.1117/12.889612

8 June 2011 Simultaneous SHG and 2PEF imaging using a new type of selective markers

E. De Meulenaere, S. Van Cleuvenbergen, S. Psilodimitrakopoulos, J. Vanderleyden, P. Loza-Alvarez, K. Clays

Proceedings Volume 8086, Advanced Microscopy Techniques II; 80861B (2011) https://doi.org/10.1117/12.889612
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

In general, second-harmonic generation (SHG) microscopy is used to image highly ordered structures in biological samples, like starch, collagen, myosin and tubulin. In an effort to expand the possible targets for SHG microscopy, a number of new fluorescent probes with high performance in SHG imaging were designed and synthesized. The design is based on an electron-rich carbazole template, functionalized with pyridinium-like acceptors, resulting in cyanine-like dyes. In this paper, we report on the linear and nonlinear optical characterization of one of these dyes and its applicability in microscopy using two-photon excited fluorescence (2PEF) and SHG to visualize the specificity of the dyes in HeLa cells.

Citation Download Citation

E. De Meulenaere, S. Van Cleuvenbergen, S. Psilodimitrakopoulos, J. Vanderleyden, P. Loza-Alvarez, and K. Clays "Simultaneous SHG and 2PEF imaging using a new type of selective markers", Proc. SPIE 8086, Advanced Microscopy Techniques II, 80861B (8 June 2011); https://doi.org/10.1117/12.889612

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