Calibration phantom for the quantification of fluorescent labels in deep skin tissue

Sebastian Beer; Ulf Maeder; Thorsten Bergmann; Peggy Schlupp; Thomas Schmidts; Johannes T. Heverhagen; Frank Runkel; Martin Fiebich

doi:10.1117/12.889973

8 June 2011 Calibration phantom for the quantification of fluorescent labels in deep skin tissue

Sebastian Beer, Ulf Maeder, Thorsten Bergmann, Peggy Schlupp, Thomas Schmidts, Johannes T. Heverhagen, Frank Runkel, Martin Fiebich

Author Affiliations +

Proceedings Volume 8086, Advanced Microscopy Techniques II; 80861L (2011) https://doi.org/10.1117/12.889973
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

A method to quantify fluorescent labels spatially resolved in scattering and absorbing samples is proposed and tested using a tissue phantom. The method works without any a priori knowledge about the optical properties of the sample. The scattering and absorption behavior of the sample is estimated by measuring reflectance from the sample simultaneously to the fluorescence. With this estimation, the attenuation of the fluorescence caused by scattering and absorption can be mathematically compensated. The method is planned to be used for evaluating skin penetrating drug carrier systems.

Citation Download Citation

Sebastian Beer, Ulf Maeder, Thorsten Bergmann, Peggy Schlupp, Thomas Schmidts, Johannes T. Heverhagen, Frank Runkel, and Martin Fiebich "Calibration phantom for the quantification of fluorescent labels in deep skin tissue", Proc. SPIE 8086, Advanced Microscopy Techniques II, 80861L (8 June 2011); https://doi.org/10.1117/12.889973

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