Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms

S. Daddi Oubekka; R. Briandet; F. Waharte; M.-P. Fontaine-Aupart; K. Steenkeste

doi:10.1117/12.889461

10 June 2011 Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms

S. Daddi Oubekka, R. Briandet, F. Waharte, M.-P. Fontaine-Aupart, K. Steenkeste

Author Affiliations +

Proceedings Volume 8087, Clinical and Biomedical Spectroscopy and Imaging II; 80871I (2011) https://doi.org/10.1117/12.889461
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

The diffusion capabilities of free fluorophores inside the heterogeneous three dimensional structure of Staphylococcus aureus biofilm were studied by an original image-based Fluorescence Recovery After Photobleaching method. The study was extended to BODIPY-vancomycin in order to better understand the mechanisms involved in the high tolerance of the bacteria embedded in a biofilm to the antibiotic.

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S. Daddi Oubekka, R. Briandet, F. Waharte, M.-P. Fontaine-Aupart, and K. Steenkeste "Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871I (10 June 2011); https://doi.org/10.1117/12.889461

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