Image analysis of free diffusing nanoparticles in vitro

Thorsten Wagner; Sven O. Luettmann; Dominic Swarat; Martin Wiemann; Hans-Gerd Lipinski

doi:10.1117/12.889620

14 June 2011 Image analysis of free diffusing nanoparticles in vitro

Thorsten Wagner, Sven O. Luettmann, Dominic Swarat, Martin Wiemann, Hans-Gerd Lipinski

Proceedings Volume 8087, Clinical and Biomedical Spectroscopy and Imaging II; 808726 (2011) https://doi.org/10.1117/12.889620
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Based on laser technology it is possible to visualize nanoparticles (NP) as light scattering objects by means of a conventional light microscope. Image series of visualized NPs were used to calculate characteristic parameters such as particle size and diffusion coefficient. Here progressive agglomeration of NP upon changes of ionic strength could be followed by repeated observation over minutes. While these analyses were based on dynamic properties of NPs, single particle paths were used to analyse particle agglomeration in more detail using image analysis methods. Together these techniques may help to better describe the behaviour of nanoparticles when used for cell culture experiments.

Citation Download Citation

Thorsten Wagner, Sven O. Luettmann, Dominic Swarat, Martin Wiemann, and Hans-Gerd Lipinski "Image analysis of free diffusing nanoparticles in vitro", Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808726 (14 June 2011); https://doi.org/10.1117/12.889620

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