Comparison Of The Appearance Of Cultured Cells Observed Using Scanning Acoustomicroscopy With That Obtained By Interference And Fluorescence Microscopy.

Jurgen Bereiter-Hahn

doi:10.1117/12.941514

3 August 1987 Comparison Of The Appearance Of Cultured Cells Observed Using Scanning Acoustomicroscopy With That Obtained By Interference And Fluorescence Microscopy.

Jurgen Bereiter-Hahn

Author Affiliations +

Proceedings Volume 0809, Scanning Imaging Technology; (1987) https://doi.org/10.1117/12.941514
Event: Fourth International Symposium on Optical and Optoelectronic Applied Sciences and Engineering, 1987, The Hague, Netherlands

Abstract

In XTH-2 cells (line derived from Xenopus laevis tadpole hearts) grown on glass, cyto-plasmic factors influencing acoustic impedance and attenuation were investigated using ultrasound of 1.6 GHz frequency. An overall correlation between the distribution of F-actin and zones with increased impedance is qualitatively assessed. Comparison of scanning acousto-microscope (SAM) images with those taken with a Mach-Zehnder type microinterferometer reveal a linear relationship between the amount of cytoplasmic mass penetrated by the sound waves, and sound attenuation. However, the attenuation coefficient for the cell periphery is considerably higher then that for the cell centre.

Citation Download Citation

Jurgen Bereiter-Hahn "Comparison Of The Appearance Of Cultured Cells Observed Using Scanning Acoustomicroscopy With That Obtained By Interference And Fluorescence Microscopy.", Proc. SPIE 0809, Scanning Imaging Technology, (3 August 1987); https://doi.org/10.1117/12.941514

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