Development of Au/Ag substrate with alternating nanosphere array for SERS-based biosensing

Chit Yaw Fu; U. S. Dinish; Kiang Wei Kho; Douglas Goh Wenda; Malini Olivo

doi:10.1117/12.890076

16 June 2011 Development of Au/Ag substrate with alternating nanosphere array for SERS-based biosensing

Chit Yaw Fu, U. S. Dinish, Kiang Wei Kho, Douglas Goh Wenda, Malini Olivo

Proceedings Volume 8090, Novel Biophotonic Techniques and Applications; 80900B (2011) https://doi.org/10.1117/12.890076
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

Nanostructure substrates are effective biosensor to spectrally differentiate multiple compounds by Surface-enhanced Raman scattering (SERS). Metal film over nanosphere (MFON) has been demonstrated to exhibit reproducible and predictable Raman enhancement. MFON can be fabricated using an economical process in which polystyrene (PS) nanospheres are self-assembled on a planar solid supports and then followed by metal coating. In this work, we investigate the MFON substrates with bimetallic coating to combine the optical-enhancing and stability features from Ag and Au layers. The SERS responses are then quantified from the resultant bimetallic structures with 2-Naphthalenethiol. We show that the bimetallic substrate of optimal Au/Ag thickness ratio renders SERS enhancement and stability exceeding those of the Au-coated MFON. Compared to Au array, the bimetallic substrate exhibits quasi-bimetallic nanoparticles of surpassing SERS (2.5 times) with enhancement factor determined to be 2×10⁷. As a proof-of-concept for biosensing in microfluidics, SERS nanotag was prepared and tested on the optimized BMFON. In addition, we propose a fabrication scheme to construct MFON with alternating sizes (100nm and 400nm) of nanosphere. At optimal proportional amount, the 100nm-spheres were packed within the gaps between the 400nm-spheres. The resultant morphology renders additional nanogaps that could possibly lead to increment in SERS enhancement.

Citation Download Citation

Chit Yaw Fu, U. S. Dinish, Kiang Wei Kho, Douglas Goh Wenda, and Malini Olivo "Development of Au/Ag substrate with alternating nanosphere array for SERS-based biosensing", Proc. SPIE 8090, Novel Biophotonic Techniques and Applications, 80900B (16 June 2011); https://doi.org/10.1117/12.890076

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available