Detection of bacterial infection with a fiber optic microendoscope

Nooman Mufti; Ying Kong; Jeffrey D. Cirillo; Kristen C. Maitland

doi:10.1117/12.896553

2 June 2011 Detection of bacterial infection with a fiber optic microendoscope

Nooman Mufti, Ying Kong, Jeffrey D. Cirillo, Kristen C. Maitland

Proceedings Volume 8092, Medical Laser Applications and Laser-Tissue Interactions V; 80920A (2011) https://doi.org/10.1117/12.896553
Event: European Conferences on Biomedical Optics, 2011, Munich, Germany

Abstract

We present the use of fiber optic microendoscopy to image bacterial infection in the skin and lungs using an animal model. The contact probe microendoscope we have constructed has a 4 μm resolution, a 750 μm field of view, and a 1 mm outer diameter. Well resolved regions of bacterial infection were imaged for subcutaneous inocula of 10⁶ to 10¹ CFU and intra-tracheal inocula of 10⁸ to 10⁶ CFU. Results reveal a linear relationship between average fluorescence and CFU, suggesting potential for using this device for quantitative analysis. Detection limits of 10⁴ CFU for skin samples and 10⁷ CFU for lung tissue were determined. In addition, bacteria were also qualitatively visible in lung tissue down to 10⁶ CFU. Confocal imaging was used to confirm the presence of bacteria in tissue samples.

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Nooman Mufti, Ying Kong, Jeffrey D. Cirillo, and Kristen C. Maitland "Detection of bacterial infection with a fiber optic microendoscope", Proc. SPIE 8092, Medical Laser Applications and Laser-Tissue Interactions V, 80920A (2 June 2011); https://doi.org/10.1117/12.896553

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KEYWORDS

Bacteria

Lung

Imaging systems

Tissues

Luminescence

Skin

Fiber optics

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