Measurement of stress and strain applied to electrochemically aligned collagen fibres by second-harmonic generation microscopy

Nobutaka Goami; Keisuke Yoshiki; Takahiro Namazu; Shozo Inoue

doi:10.1117/12.894151

16 September 2011 Measurement of stress and strain applied to electrochemically aligned collagen fibres by second-harmonic generation microscopy

Nobutaka Goami, Keisuke Yoshiki, Takahiro Namazu, Shozo Inoue

Author Affiliations +

Proceedings Volume 8113, Linear and Nonlinear Optics of Organic Materials XI; 81130E (2011) https://doi.org/10.1117/12.894151
Event: SPIE Photonic Devices + Applications, 2011, San Diego, California, United States

Abstract

In this study, we developed a novel strain measurement technique for electrochemically aligned collagen (ELAC) fibres using second harmonic generation (SHG) microscopy. The ELAC fibres were prepared by a typical electrochemical method and were subjected to cross-linking. For comparison with natural collagen fibres, polarization dependency of the prepared ELAC fibres and that of a human Achilles' tendon were evaluated. The results showed that, because of crosslinking, the ELAC fibres exhibit polarization dependency similar to that of the tendon but only in a region close to the tendon. The relationship between SHG and the applied strain was determined by a combination of SHG microscopy and tensile tests. The SHG from the ELAC fibres changed in the high strain region because of the applied stress.

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Nobutaka Goami, Keisuke Yoshiki, Takahiro Namazu, and Shozo Inoue "Measurement of stress and strain applied to electrochemically aligned collagen fibres by second-harmonic generation microscopy", Proc. SPIE 8113, Linear and Nonlinear Optics of Organic Materials XI, 81130E (16 September 2011); https://doi.org/10.1117/12.894151

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KEYWORDS

Polarization

Second-harmonic generation

Collagen

Microscopy

Signal detection

Tissues

Harmonic generation

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