Development of a specimen holder combined with ultra thin film laser plasma x-ray source for compact contact-type soft x-ray microscope to observe hydrated living biological cells

Masahiko Ishino; Masataka Kado; Kunio Shinohara; Yoshimasa Yamamoto; Itaru Hirai; Maki Kishimoto; Masaharu Nishikino; Noboru Hasegawa; Satoshi Tamotsu; Keiko Yasuda; Tetsuya Kawachi

doi:10.1117/12.892864

28 September 2011 Development of a specimen holder combined with ultra thin film laser plasma x-ray source for compact contact-type soft x-ray microscope to observe hydrated living biological cells

Masahiko Ishino, Masataka Kado, Kunio Shinohara, Yoshimasa Yamamoto, Itaru Hirai, Maki Kishimoto, Masaharu Nishikino, Noboru Hasegawa, Satoshi Tamotsu, Keiko Yasuda, Tetsuya Kawachi

Author Affiliations +

Proceedings Volume 8139, Advances in X-Ray/EUV Optics and Components VI; 81390R (2011) https://doi.org/10.1117/12.892864
Event: SPIE Optical Engineering + Applications, 2011, San Diego, California, United States

Abstract

Ultra thin gold films having a thickness of 20-30 nm are favorable laser plasma targets for a soft x-ray microscopy, because the ultra thin films emit intense soft x-rays at the wavelength of water window region from the rear side with respect to the surface irradiated with short pulse laser. Using rear side emissions, the distance between the x-ray source and the specimens can be reduced so that the x-ray flux on specimens increases. In addition, the microscope system can be designed to be compact when the specimen holder and x-ray source are combined in one piece. In the present study, the biological specimen holder combined with a gold ultra thin film plasma target has been developed for a contact-type soft x-ray microscope. This x-ray microscope system needs not any x-ray optics such as a condenser and/or an objective optics which causes a decrease in x-ray photons for imaging. Specimen holder equipped with the plasma target keeps biological specimens at wet condition in vacuum. In this study, x-ray images of hydrated living cells (MH-S mouse alveolar macrophage cell line) have been obtained successfully by use of the newly developed specimen holder. These experimental results reveal that the soft x-ray image can be taken safely. Specimen holder combined with plasma x-ray source will be a key component of a compact soft x-ray microscope using in a laboratory.

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Masahiko Ishino, Masataka Kado, Kunio Shinohara, Yoshimasa Yamamoto, Itaru Hirai, Maki Kishimoto, Masaharu Nishikino, Noboru Hasegawa, Satoshi Tamotsu, Keiko Yasuda, and Tetsuya Kawachi "Development of a specimen holder combined with ultra thin film laser plasma x-ray source for compact contact-type soft x-ray microscope to observe hydrated living biological cells", Proc. SPIE 8139, Advances in X-Ray/EUV Optics and Components VI, 81390R (28 September 2011); https://doi.org/10.1117/12.892864

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