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13 October 2011 A new optical method for a fast and simple detection of ephedrine
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In this work we describe the synthesis of a new ephedrine derivative with a carbon linker featuring an amino reactive group, and its conjugation to the glutamine binding protein (GlnBP) from E. coli as a carrier protein for the production of polyclonal antibodies in rabbits against ephedrine. Proof-of-principle results that an efficient SPR-based indirect competitive immunoassay for the detection and quantification of ephedrine are presented. The detection limit of this assay was found to be about 33ng/ml.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Antonio Varriale, Maria Staiano, Maria Strianese, Vincenzo Marzullo, Giuseppe Ruggiero, Alberto Secchi, Massimiliano Dispenza, Anna Maria Fiorello, and Sabato D'Auria "A new optical method for a fast and simple detection of ephedrine", Proc. SPIE 8189, Optics and Photonics for Counterterrorism and Crime Fighting VII; Optical Materials in Defence Systems Technology VIII; and Quantum-Physics-based Information Security, 81890M (13 October 2011);

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