All-optical control of neuronal function via optical delivery of light-sensitive proteins and optogenetic stimulation

Alex Villalobos; Ling Gu; Samarendra Mohanty

doi:10.1117/12.916751

17 February 2012 All-optical control of neuronal function via optical delivery of light-sensitive proteins and optogenetic stimulation

Alex Villalobos, Ling Gu, Samarendra Mohanty

Author Affiliations +

Proceedings Volume 8207, Photonic Therapeutics and Diagnostics VIII; 82076B (2012) https://doi.org/10.1117/12.916751
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

While pulsed laser beams have been used for stimulation of neurons, cellular specificity during optical stimulation is achieved by photo-sensitization of genetically-targeted cells by optogenetic means. However, till date, the process of optogenetic-sensitization primarily involves use of viral vectors. In rare occasions, electroporation has been used. Here, we report an all-optical method in which pulsed laser beam is used for delivery of genes, encoding optogenetic probes, to spatially-targeted cells, followed by optogenetic stimulation and optical detection of the activation process. Use of laser microbeam enabled highly precise spatially-patterned delivery of optogenes, as confirmed by expression of conjugated fluorescent protein. Light-activation of opsin-expressing cells was confirmed by calcium-imaging. The laser-assisted expression of optogenetic probes in spatially-targeted regions in combination with light-assisted activation and optical detection of neural activity will help in better understanding of the neuronal circuitry.

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Alex Villalobos, Ling Gu, and Samarendra Mohanty "All-optical control of neuronal function via optical delivery of light-sensitive proteins and optogenetic stimulation", Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82076B (17 February 2012); https://doi.org/10.1117/12.916751

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