Lipid-encapsulation of surface enhanced Raman scattering (SERS) nanoparticles and targeting to chronic lymphocytic leukemia (CLL) cells

Shell Y. Ip; Christina M. MacLaughlin; Nisa Mullaithilaga; Michelle Joseph; Samantha Wala; Chen Wang; Gilbert C. Walker

doi:10.1117/12.909179

31 January 2012 Lipid-encapsulation of surface enhanced Raman scattering (SERS) nanoparticles and targeting to chronic lymphocytic leukemia (CLL) cells

Shell Y. Ip, Christina M. MacLaughlin, Nisa Mullaithilaga, Michelle Joseph, Samantha Wala, Chen Wang, Gilbert C. Walker

Author Affiliations +

Proceedings Volume 8212, Frontiers in Biological Detection: From Nanosensors to Systems IV; 821204 (2012) https://doi.org/10.1117/12.909179
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

60 nm diameter gold nanoparticles (AuNP) were coated with a ternary mixture of lipids and targeted to human lymphocytes. Previously, the versatility, stability and ease of application of the lipid coating was demonstrated by the incorporation of three classes of Raman-active species. In the present study, lipid encapsulated AuNPs were conjugated to two targeting species, namely whole antibodies and antibody fragments (Fab), by two methods. Furthermore, in vitro targeting of lipid-encapsulated Au nanoparticles to patient-derived chronic lymphocytic leukemia (CLL) cells was demonstrated by Raman spectroscopy, Raman mapping, and darkfield microscopy. These results further demonstrate the versatility of the lipid layer for imparting stability, SERS activity, and targeting capability, which make these particles promising candidates for biodiagnostics.

Citation Download Citation

Shell Y. Ip, Christina M. MacLaughlin, Nisa Mullaithilaga, Michelle Joseph, Samantha Wala, Chen Wang, and Gilbert C. Walker "Lipid-encapsulation of surface enhanced Raman scattering (SERS) nanoparticles and targeting to chronic lymphocytic leukemia (CLL) cells", Proc. SPIE 8212, Frontiers in Biological Detection: From Nanosensors to Systems IV, 821204 (31 January 2012); https://doi.org/10.1117/12.909179

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