Laser based enhancement of susceptibility of bacteria to antibiotic

Yana Reznick; Ehud Banin; Anat Lipovsky; Rachel Lubart; Zeev Zalevsky

doi:10.1117/12.901520

14 February 2012 Laser based enhancement of susceptibility of bacteria to antibiotic

Yana Reznick, Ehud Banin, Anat Lipovsky, Rachel Lubart, Zeev Zalevsky

Proceedings Volume 8224, Biophotonics and Immune Responses VII; 82240K (2012) https://doi.org/10.1117/12.901520
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Our objective is to test the effect of pulsed (Q-switched) and continuous wave (CW) laser light at wavelength of 532nm on the viability of free-living stationary phase bacteria with and without gentamicin (an antibiotic) treatment. Free living stationary phase gram negative bacteria (Pseudomonas aeruginosa strain PAO1) was immersed in Luria Broth (LB) solution and exposed to Q-switched and CW lasers with and without the addition of the antibiotic gentamicin. Cell viability was determined at different time points. Laser treatment alone did not reduce cell viability compared to untreated control and the gentamicin treatment alone only resulted in a 0.5 log reduction in the viable count for P. aeruginosa. The combined laser and gentamicin treatment, however, resulted in a synergistic effect and viability was reduced by 8 log's for P. aeruginosa PAO1.

Citation Download Citation

Yana Reznick, Ehud Banin, Anat Lipovsky, Rachel Lubart, and Zeev Zalevsky "Laser based enhancement of susceptibility of bacteria to antibiotic", Proc. SPIE 8224, Biophotonics and Immune Responses VII, 82240K (14 February 2012); https://doi.org/10.1117/12.901520

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