Spectrally resolved visualization of fluorescent dyes permeating into skin

Ulf Maeder; Thorsten Bergmann; Sebastian Beer; Jan Michael Burg; Thomas Schmidts; Frank Runkel; Martin Fiebich

doi:10.1117/12.907815

9 February 2012 Spectrally resolved visualization of fluorescent dyes permeating into skin

Ulf Maeder, Thorsten Bergmann, Sebastian Beer, Jan Michael Burg, Thomas Schmidts, Frank Runkel, Martin Fiebich

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 822523 (2012) https://doi.org/10.1117/12.907815
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We present a spectrally resolved confocal imaging approach to qualitatively asses the overall uptake and the penetration depth of fluorescent dyes into biological tissue. We use a confocal microscope with a spectral resolution of 5 nm to measure porcine skin tissue after performing a Franz-Diffusion experiment with a submicron emulsion enriched with the fluorescent dye Nile Red. The evaluation uses linear unmixing of the dye and the tissue autofluorescence spectra. The results are combined with a manual segmentation of the skin's epidermis and dermis layers to assess the penetration behavior additionally to the overall uptake. The diffusion experiments, performed for 3h and 24h, show a 3-fold increased dye uptake in the epidermis and dermis for the 24h samples. As the method is based on spectral information it does not face the problem of superimposed dye and tissue spectra and therefore is more precise compared to intensity based evaluation methods.

Citation Download Citation

Ulf Maeder, Thorsten Bergmann, Sebastian Beer, Jan Michael Burg, Thomas Schmidts, Frank Runkel, and Martin Fiebich "Spectrally resolved visualization of fluorescent dyes permeating into skin", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 822523 (9 February 2012); https://doi.org/10.1117/12.907815

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