Spatially resolved recording of transient fluorescence-lifetime effects by line-scanning TCSPC

Wolfgang Becker; Bertram Su; Axel Bergmann

doi:10.1117/12.908663

9 February 2012 Spatially resolved recording of transient fluorescence-lifetime effects by line-scanning TCSPC

Wolfgang Becker, Bertram Su, Axel Bergmann

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82260C (2012) https://doi.org/10.1117/12.908663
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We present a technique that records transient effects in the fluorescence lifetime of a sample with spatial resolution along a one-dimensional scan. The technique is based on scanning a sample with a high-frequency pulsed laser beam, and building up a photon distribution over the distance along the scan, the arrival times of the photons after the excitation pulses, and the time after a stimulation of the sample. The maximum resolution at which lifetime changes can be recorded is given by the line scan time. With repetitive stimulation and triggered accumulation transient lifetime effects can be resolved at a resolution of about one millisecond.

Citation Download Citation

Wolfgang Becker, Bertram Su, and Axel Bergmann "Spatially resolved recording of transient fluorescence-lifetime effects by line-scanning TCSPC", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260C (9 February 2012); https://doi.org/10.1117/12.908663

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