Multiwavelength FLIM: new concept for fluorescence diagnosis

Angelika Rück; S. Lorenz; Carmen Hauser; S. Mosch; S. Kalinina

doi:10.1117/12.906620

9 February 2012 Multiwavelength FLIM: new concept for fluorescence diagnosis

Angelika Rück, S. Lorenz, Carmen Hauser, S. Mosch, S. Kalinina

Author Affiliations +

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82260G (2012) https://doi.org/10.1117/12.906620
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Fluorescence guided tumor resection is very well accepted in the case of bladder cancer and brain tumor, respectively. However, false positive results are one of the major problems, which will make the discrimination between tumor tissue and inflammation difficult. In contrast fluorescence lifetime imaging (FLIM) and especially spectral resolved FLIM (SLIM) can significantly improve the analysis. The fluorescence decay of a fluorophore in many cases does not show a simple monoexponential profile. A very complex situation arises, when more than one compound has to be analyzed. This could be the case when endogenous fluorophores of living cells and tissues have to be discriminated to identify oxidative metabolic changes. Other examples are PDT, when different photosensitizer metabolites are observed simultaneously. In those cases a considerable improvement could be achieved when time-resolved and spectral-resolved techniques are simultaneously incorporated. Within this presentation the principles of spectral and time-resolved fluorescence imaging will be discussed. Successful applications as autofluorescence and 5-ALA induced porphyrin fluorescence will be described in more detail.

Citation Download Citation

Angelika Rück, S. Lorenz, Carmen Hauser, S. Mosch, and S. Kalinina "Multiwavelength FLIM: new concept for fluorescence diagnosis", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82260G (9 February 2012); https://doi.org/10.1117/12.906620

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