Clinical multiphoton and CARS microscopy

H. G. Breunig; M. Weinigel; M. E. Darvin; J. Lademann; K. König

doi:10.1117/12.908187

9 February 2012 Clinical multiphoton and CARS microscopy

H. G. Breunig, M. Weinigel, M. E. Darvin, J. Lademann, K. König

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 822623 (2012) https://doi.org/10.1117/12.908187
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We report on clinical CARS imaging of human skin in vivo with the certified hybrid multiphoton tomograph CARSDermaInspect. The CARS-DermaInspect provides simultaneous imaging of non-fluorescent intradermal lipid and water as well as imaging of two-photon excited fluorescence from intrinsic molecules. Two different excitation schemes for CARS imaging have been realized: In the first setup, a combination of fs oscillator and optical parametric oscillator provided fs-CARS pump and Stokes pulses, respectively. In the second setup a fs oscillator was combined with a photonic crystal fiber which provided a broadband spectrum. A spectral range out of the broadband-spectrum was selected and used for CARS excitation in combination with the residual fs-oscillator output. In both setups, in addition to CARS, single-beam excitation was used for imaging of two-photon excited fluorescence and second harmonic generation signals. Both CARS-excitation systems were successfully used for imaging of lipids inside the skin in vivo.

Citation Download Citation

H. G. Breunig, M. Weinigel, M. E. Darvin, J. Lademann, and K. König "Clinical multiphoton and CARS microscopy", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822623 (9 February 2012); https://doi.org/10.1117/12.908187

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