Recommendations for the design and the installation of large laser scanning microscopy systems

P. Johannes Helm

doi:10.1117/12.906040

13 February 2012 Recommendations for the design and the installation of large laser scanning microscopy systems

P. Johannes Helm

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82262M (2012) https://doi.org/10.1117/12.906040
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Laser Scanning Microscopy (LSM) has since the inventions of the Confocal Scanning Laser Microscope (CLSM) and the Multi Photon Laser Scanning Microscope (MPLSM) developed into an essential tool in contemporary life science and material science. The market provides an increasing number of turn-key and hands-off commercial LSM systems, un-problematic to purchase, set up and integrate even into minor research groups. However, the successful definition, financing, acquisition, installation and effective use of one or more large laser scanning microscopy systems, possibly of core facility character, often requires major efforts by senior staff members of large academic or industrial units. Here, a set of recommendations is presented, which are helpful during the process of establishing large systems for confocal or non-linear laser scanning microscopy as an effective operational resource in the scientific or industrial production process. Besides the description of technical difficulties and possible pitfalls, the article also illuminates some seemingly "less scientific" processes, i.e. the definition of specific laboratory demands, advertisement of the intention to purchase one or more large systems, evaluation of quotations, establishment of contracts and preparation of the local environment and laboratory infrastructure.

Citation Download Citation

P. Johannes Helm "Recommendations for the design and the installation of large laser scanning microscopy systems", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82262M (13 February 2012); https://doi.org/10.1117/12.906040

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KEYWORDS

Optical benches

Laser scanners

Microscopy

Confocal microscopy

Laser systems engineering

Microscopes

Laser safety

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