Long-distance fluorescence lifetime imaging with stimulated emission and electronic time delay

Po-Yen Lin; Shin-Shian Lee; Chia-Seng Chang; Fu-Jen Kao

doi:10.1117/12.907632

9 February 2012 Long-distance fluorescence lifetime imaging with stimulated emission and electronic time delay

Po-Yen Lin, Shin-Shian Lee, Chia-Seng Chang, Fu-Jen Kao

Proceedings Volume 8226, Multiphoton Microscopy in the Biomedical Sciences XII; 82262T (2012) https://doi.org/10.1117/12.907632
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

In this work, long-distance fluorescence lifetime imaging is realized by stimulated emission and electronic time delay control. Spatial coherence, as a result of stimulated emission, provides unattenuated fluorescence detection at long distance with low NA optics. The electronic trigger also provides a feasible way to change the pulse separation and probe the fluorescence lifetime in nano-second range. The lifetime of fluorophores is determined by measuring the stimulated emission signal at various the time delays between excitation and stimulation pulses. The characteristics of stimulated emission are investigated and the saturation condition is also presented.

Citation Download Citation

Po-Yen Lin, Shin-Shian Lee, Chia-Seng Chang, and Fu-Jen Kao "Long-distance fluorescence lifetime imaging with stimulated emission and electronic time delay", Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82262T (9 February 2012); https://doi.org/10.1117/12.907632

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