Improved contrast by modal illumination in scanning reflectance confocal microscopy

Christopher Glazowski; James Zavislan

doi:10.1117/12.906438

2 February 2012 Improved contrast by modal illumination in scanning reflectance confocal microscopy

Proceedings Volume 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX; 822715 (2012) https://doi.org/10.1117/12.906438
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Scanning reflectance confocal microscopy (SRCM) is a flexible technology that provides cellular resolution images of tissue morphology with tailored resolutions and fields of view. However, how accurately an object is represented, in other words its fidelity, is critical in medical imaging and is not represented simply by optical resolution. In this work we characterize the SRCMs fidelity of images derived within turbid media. We present theoretical and experimental results showing the improved fidelity when using modal illumination. We investigated the use of TEM10 illumination and a novel implementation of Nomarski differential-interference-contrast (DIC). Using a repeatable, stable turbid phantom the system fidelity was characterized.

Citation Download Citation

Christopher Glazowski and James Zavislan "Improved contrast by modal illumination in scanning reflectance confocal microscopy", Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 822715 (2 February 2012); https://doi.org/10.1117/12.906438

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