You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither SPIE nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the SPIE website.
2 February 2012Multimodal light-sheet microscopy for fluorescence live imaging
Light-sheet microscopy, it is known as single plane illumination microscope (SPIM), is a fluorescence imaging
technique which can avoid phototoxic effects to living cells and gives high contrast and high spatial resolution by optical
sectioning with light-sheet illumination in developmental biology. We have been developed a multifunctional light-sheet
fluorescence microscopy system with a near infrared femto-second fiber laser, a high sensitive image sensor and a high
throughput spectrometer. We performed that multiphoton fluorescence images of a transgenic fish and a mouse embryo
were observed on the light-sheet microscope. As the results, two photon images with high contrast and high spatial
resolution were successfully obtained in the microscopy system. The system has multimodality, not only mutiphoton
fluorescence imaging, but also hyperspectral imaging, which can be applicable to fluorescence unmixing analysis and
Raman imaging. It enables to obtain high specific and high throughput molecular imaging in vivo and in vitro.
The alert did not successfully save. Please try again later.
Y. Oshima, H. Kajiura-Kobayashi, S. Nonaka, "Multimodal light-sheet microscopy for fluorescence live imaging," Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82271H (2 February 2012); https://doi.org/10.1117/12.907937