Concentration and detection of bacteria in virtual environmental samples based on non-immunomagnetic separation and quantum dots by using a laboratory-made system

Zhi Cheng; Taihu Wu; Feng Chen; Yaohua Du; Biao Gu; Chao Li; Zijian Yang

doi:10.1117/12.907367

2 February 2012 Concentration and detection of bacteria in virtual environmental samples based on non-immunomagnetic separation and quantum dots by using a laboratory-made system

Zhi Cheng, Taihu Wu, Feng Chen, Yaohua Du, Biao Gu, Chao Li, Zijian Yang

Author Affiliations +

Proceedings Volume 8231, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII; 82310Y (2012) https://doi.org/10.1117/12.907367
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

This study investigated a method that simultaneously detects three bacteria, Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus via an approach that combines un-immunized magnetic nanoparticles for the enrichment and antibody-conjugated quantum dots (QDs) as fluorescence markers, by using a laboratory-made system. In the enrichment procedure, the un-immunized superparamagnetic polymer nanoparticles and the three bacteria formed "beadcell" complex. Magnetic nanoparticles with different size were used and some interferents were added into the bacteria suspension respectively to check the influence on concentration efficiency. In the immuno-fluorescence labeling procedure, QDs with different emission wavelenghs were immobilized with antibody. Antibody conjugated QDs capture the bacteria selectively and specifically so that "sandwich" complex were formed. The suspension of the labeled bacteria was trickled onto a microporous membrane. A 450nm semiconductor laser was used as a part of the laboratory-made system to excite the QDs. Three PMT detectors were utilized to detect the fluorescence intensity. These un-immunized magnetic nanoparticles can be applied in nonspecific separation and enrichment of bacteria from environmental samples, and this method, of which the detection procedures are completed within 2 h, can be applied to the cost-effective and rapid detecting of bacterial contamination.

Citation Download Citation

Zhi Cheng, Taihu Wu, Feng Chen, Yaohua Du, Biao Gu, Chao Li, and Zijian Yang "Concentration and detection of bacteria in virtual environmental samples based on non-immunomagnetic separation and quantum dots by using a laboratory-made system", Proc. SPIE 8231, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VIII, 82310Y (2 February 2012); https://doi.org/10.1117/12.907367

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