Fluorescent diamond nanoparticle as a probe of intracellular traffic in primary neurons in culture

Xuan Loc Le; Aude-Marie Lepagnol-Bestel; Marie-Pierre Adam; Alice Thomas; Géraldine Dantelle; Cheng-Chun Chang; Nitin Mohan; Huan-Cheng Chang; François Treussart; Michel Simonneau

doi:10.1117/12.907298

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2 February 2012 Fluorescent diamond nanoparticle as a probe of intracellular traffic in primary neurons in culture

Xuan Loc Le, Aude-Marie Lepagnol-Bestel, Marie-Pierre Adam, Alice Thomas, Géraldine Dantelle, Cheng-Chun Chang, Nitin Mohan, Huan-Cheng Chang, François Treussart, Michel Simonneau

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Proceedings Volume 8232, Colloidal Nanocrystals for Biomedical Applications VII; 823203 (2012) https://doi.org/10.1117/12.907298
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Neurons display dendritic spines plasticity and morphology anomalies in numerous psychiatric and neurodegenerative diseases. These changes are associated to abnormal dendritic traffic that can be evidenced by fluorescence microscopy. As a fluorescent probe we propose to use fluorescent diamond nanoparticles with size of < 50 nm. Color centers embedded inside the diamond nanoparticles are perfectly photostable emitters allowing for long-term tracking. Nanodiamond carbon surface is also well suited for biomolecule functionalization to target specific cellular compartments. We show that fluorescent nanodiamonds can be spontaneously internalized in neurons in culture and imaged by confocal and Total Internal Reflection (TIRF) microscopy with a high signal over background ratio.

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Xuan Loc Le, Aude-Marie Lepagnol-Bestel, Marie-Pierre Adam, Alice Thomas, Géraldine Dantelle, Cheng-Chun Chang, Nitin Mohan, Huan-Cheng Chang, François Treussart, and Michel Simonneau "Fluorescent diamond nanoparticle as a probe of intracellular traffic in primary neurons in culture", Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 823203 (2 February 2012); https://doi.org/10.1117/12.907298

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