Magnetic vesicles as MRI-trackable biogenic nanovectors

Amanda K. Andriola Silva; Nathalie Luciani; Florence Gazeau; Claire Wilhelm

doi:10.1117/12.906783

2 February 2012 Magnetic vesicles as MRI-trackable biogenic nanovectors

Amanda K. Andriola Silva, Nathalie Luciani, Florence Gazeau, Claire Wilhelm

Proceedings Volume 8232, Colloidal Nanocrystals for Biomedical Applications VII; 82320Y (2012) https://doi.org/10.1117/12.906783
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Magnetic labeling renders cells MRI-detectable which provides attractive solutions for tracking the fate of a transplanted cell population. Understanding the interplay of magnetic nanoparticles and cells is then an important point that should not be neglected. Here we show that in the condition of food starvation, macrophage cells emit vesicles containing nanoparticles. First, we inferred the intracellular iron oxide load from the magnetophoretic velocity of cells at a calibrated magnetic field gradient. After magnetic labeling and culture in stress conditions, the intracellular iron oxide load was once more determined and a detectable difference was observed before and after stress. Moreover, we identified in the stress conditioned medium membrane vesicle structures carrying magnetic particles. Besides pointing out the role of cell-derived vesicles in the sequestration of the intracellular magnetic label, experiments also demonstrated that vesicles were able to chaperone the magnetic cargo into naïve cells.

Citation Download Citation

Amanda K. Andriola Silva, Nathalie Luciani, Florence Gazeau, and Claire Wilhelm "Magnetic vesicles as MRI-trackable biogenic nanovectors", Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 82320Y (2 February 2012); https://doi.org/10.1117/12.906783

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