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2 February 2012 Characterization and bioactivity study of nanohydroxyapatite on superhydrophilic vertically aligned carbon nanotubes using optical techniques
Joao Anderson Ferreira Irineu, Teresa Cristina Marsi, Tiago Goncalves Santos, Ana Maria Espirito Santo, Joao Lucas Rangel, Ursula A. Mengui, Airton Abrahao Martin, Evaldo Jose Corat, Fernanda Roberta Marciano, Anderson Oliveira Lobo
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Proceedings Volume 8232, Colloidal Nanocrystals for Biomedical Applications VII; 82321K (2012) https://doi.org/10.1117/12.907093
Event: SPIE BiOS, 2012, San Francisco, California, United States
Abstract
Vertically-aligned multi-walled carbon nanotubes (VACNT) is of particular interest in regenerative medicine. Templateinduced hydroxyapatite (HA) has broad prospects in applied fields of bone regenerative medicine. Thus, it becomes very attractive a combination these two excellent materials to bone tissue engineering applications. In this study the HA/VACNT nanocomposites were used as scaffolds to Human osteoblast cells culture. Superhydrophilic VACNT films were obtained by CVD method and funcionalized by oxygen plasma. The fabrication of HA/VACNT nanocomposites was performed with a direct electrodeposition of the thin HA films on the VACNT films. The bioactivity and biomineralization in vitro process of superhydrophilic HA/VACNT nanocomposites were investigated using simulated body fluid (SBF) and optical techniques. The characterization of of HA/VACNT nanocomposites was performed before and after soaking 21 days in SBF and compared to superydrophilic VACNT films. Fourier transform infrared spectroscopy, micro X-ray fluorescence spectrometer by energy-dispersive and X-ray difractogram were employed to investigate the structural and chemical properties. The morphology was investigated by FEG-SEM analysis. After 21 days was identified that others biological apatites were formed only on HA/VACNT nanocomposites. Optical techniques showing a powerful tool to characterizated and investigated the bioactivity in vitro process. These findings were very atractive to application of this new nanocomposite to bone tissue regeneration.
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Joao Anderson Ferreira Irineu, Teresa Cristina Marsi, Tiago Goncalves Santos, Ana Maria Espirito Santo, Joao Lucas Rangel, Ursula A. Mengui, Airton Abrahao Martin, Evaldo Jose Corat, Fernanda Roberta Marciano, and Anderson Oliveira Lobo "Characterization and bioactivity study of nanohydroxyapatite on superhydrophilic vertically aligned carbon nanotubes using optical techniques", Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 82321K (2 February 2012); https://doi.org/10.1117/12.907093
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KEYWORDS
Nanocomposites
Apatite
Bone
Biological research
Calcium
Carbon nanotubes
Calcite
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